The Dipeptide-Ome in Health and Disease – Unraveling far-reaching impact of neglected metabolites (Diptome)
A new comprehensive dipeptide-metabolome quantification method via UPLC-MS/MS
Based on the twenty proteogenic amino acids available for metabolism, 400 dipeptides should be present in organisms in theory. An interinstitutional Alliance group has now developed and validated a comprehensive dipeptide-metabolome quantification method via UPLC-MS/MS. Due to the low abundance of (several) dipeptides this requires the sensitivity of state-of-the-art tandem-quadrupole mass spectrometers as provided via the QTRAP 6500+ system. They improved and enhanced for this project an already published method enabling the analysis of 36 dipeptides to cover meanwhile with the newly developed version over 200 different dipeptides. They detected numerous dipeptides in mouse and human tissue and were able to show specific dipeptide patterns for different interventions. The dipeptidome will be correlated with clinical parameters to learn more about the role of dipeptides in diseases.
Coordination: Verena Peters, Medical Faculty Heidelberg, Heidelberg University + Claus Peter Schmitt, Medical Faculty Heidelberg, Heidelberg University
Team members: Dan Wen, Medical Faculty Heidelberg, Heidelberg University, Sven Garbade, Medical Faculty Heidelberg, Heidelberg University, Katharina Küper, Medical Faculty Heidelberg, Heidelberg University, Stefan Niedermaier, Heidelberg University & Gernot Poschet, Heidelberg University
Platform involved: Metabolomics Core Technology Platform, Heidelberg University